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PPP1R8 polyclonal antibody

  • Catalog # : PAB6447
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of PPP1R8.
  • Immunogen:
  • A synthetic peptide corresponding to C terminus of human PPP1R8.
  • Sequence:
  • C-EAWPGKKPTPSLLI
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 13.3, 38.5, 22.7
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize all three human isoforms of this protein.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Western Blot (1-2 ug/mL)br>Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-3.8 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • PPP1R8 polyclonal antibody (Cat # PAB6447) staining (2 ug/mL) of NCI-H460 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • PPP1R8 polyclonal antibody (Cat # PAB6447) (3.8 ug/mL) staining of paraffin embedded human thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5511
  • Protein Accession#:
  • NP_002704;NP_054829;NP_612568
  • Gene Name:
  • PPP1R8
  • Gene Alias:
  • ARD-1,ARD1,NIPP-1,NIPP1,PRO2047
  • Gene Description:
  • protein phosphatase 1, regulatory (inhibitor) subunit 8
  • Gene Summary:
  • This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000003847,OTTHUMP00000003848,OTTHUMP00000003849,OTTHUMP00000044938,RNase E,activator of RNA decay,nuclear inhibitor of protein phosphatase-1,nuclear subunit of PP-1,protein phosphatase 1 regulatory inhibitor subunit 8,protein phosphatase 1 regula
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