PES1 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PES1.
Immunogen
A synthetic peptide corresponding to N terminus of human PES1.
Sequence
GGLEKKKYERGSA-C
Host
Goat
Theoretical MW (kDa)
68
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(2.5-3.8 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
PES1 polyclonal antibody (Cat # PAB6415) staining (1 ug/mL) of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PES1 polyclonal antibody (Cat # PAB6415) (3.8 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PES1
Entrez GeneID
23481Protein Accession#
NP_055118Gene Name
PES1
Gene Alias
PES
Gene Description
pescadillo homolog 1, containing BRCT domain (zebrafish)
Omim ID
605819Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is abnormally elevated in malignant tumors of astrocytic origin. It is a strongly conserved gene containing a BRCT domain that is essential for the activity of this gene product. The gene plays a crucial role in cell proliferation and may be necessary for oncogenic transformation and tumor progression. [provided by RefSeq
Other Designations
pescadillo homolog 1, containing BRCT domain
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Interactome
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Publication Reference
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Pescadillo interacts with the cadmium response element of the human heme oxygenase-1 promoter in renal epithelial cells.
Sikorski EM, Uo T, Morrison RS, Agarwal A.
The Journal of Biological Chemistry 2006 Jun; 281(34):24423.
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Pescadillo interacts with the cadmium response element of the human heme oxygenase-1 promoter in renal epithelial cells.
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