USP11 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
USP11 polyclonal antibody (Cat # PAB6408) staining (1 ug/mL) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of USP11.
Immunogen
A synthetic peptide corresponding to human USP11.
Sequence
CSSPPSSEFMDVN
Host
Goat
Theoretical MW (kDa)
110
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
USP11 polyclonal antibody (Cat # PAB6408) staining (1 ug/mL) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — USP11
Entrez GeneID
8237Protein Accession#
NP_004642Gene Name
USP11
Gene Alias
UHX1
Gene Description
ubiquitin specific peptidase 11
Omim ID
300050Gene Ontology
HyperlinkGene Summary
Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This gene encodes a deubiquitinating enzyme which lies in a gene cluster on chromosome Xp11.23 [provided by RefSeq
Other Designations
deubiquitinating enzyme 11|ubiquitin carboxyl-terminal hydrolase, X-linked|ubiquitin specific protease 11|ubiquitin thiolesterase 11|ubiquitin-specific processing protease 11
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Interactome
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Publication Reference
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Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM.
Ideguchi H, Ueda A, Tanaka M, Yang J, Tsuji T, Ohno S, Hagiwara E, Aoki A, Ishigatsubo Y.
The Biochemical Journal 2002 Oct; 367(Pt 1):87.
Application：IF, WB, Human, HeLa cells.
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Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM.
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