IFIH1 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
IFIH1 polyclonal antibody (Cat # PAB6401) staining (10 ug/mL) of paraffin embedded human pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Flow Cytometry
IFIH1 polyclonal antibody (Cat # PAB6401) Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of IFIH1.
Immunogen
A synthetic peptide corresponding to N-terminus of human IFIH1.
Sequence
SNGYSTDENFRYL-C
Host
Goat
Theoretical MW (kDa)
117
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Flow Cytometry (10 ug/mL)
Immunohistochemistry (10 ug/mL)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
IFIH1 polyclonal antibody (Cat # PAB6401) staining (10 ug/mL) of paraffin embedded human pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
IFIH1 polyclonal antibody (Cat # PAB6401) Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — IFIH1
Entrez GeneID
64135Protein Accession#
NP_071451.2Gene Name
IFIH1
Gene Alias
Hlcd, IDDM19, MDA-5, MDA5, MGC133047
Gene Description
interferon induced with helicase C domain 1
Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. [provided by RefSeq
Other Designations
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide|helicard|melanoma differentiation associated protein-5
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Interactome
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Disease
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Publication Reference
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Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling.
Liao S, Bao X, Liu T, Lai S, Li K, Garofalo RP, Casola A.
The Journal of General Virology 2008 Aug; 89(Pt 8):1978.
Application：WB, Human, A-549 cells.
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Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis.
Bao X, Sinha M, Liu T, Hong C, Luxon BA, Garofalo RP, Casola A.
Virology 2008 Apr; 374(1):114.
Application：WB, Human, A-549 cells.
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mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties.
Dong-chul Kang, Rahul V Gopalkrishnan, Qingping Wu, Eckhard Jankowsky, Anna Marie Pyle, Paul B Fisher.
PNAS 2002 Jan; 99(2):637.
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Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling.
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