RNF141 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
RNF141 polyclonal antibody (Cat # PAB6397) staining (1 ug/mL) of human testis lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RNF141.
Immunogen
A synthetic peptide corresponding to human RNF141.
Sequence
C-LNMADEAGQPHRP
Host
Goat
Theoretical MW (kDa)
25.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
RNF141 polyclonal antibody (Cat # PAB6397) staining (1 ug/mL) of human testis lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RNF141
Entrez GeneID
50862Protein Accession#
NP_057506Gene Name
RNF141
Gene Alias
MGC8715, ZFP26, ZNF230
Gene Description
ring finger protein 141
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-DNA and protein-protein interactions. Abundant expression of this gene was found in the testicular tissue of fertile men, but was not detected in azoospermic patients. Studies of the mouse counterpart suggest that this gene may function as a testis specific transcription factor during spermatogenesis. [provided by RefSeq
Other Designations
C3HC4-like zinc finger protein
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Interactome
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Publication Reference
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The shorter zinc finger protein ZNF230 gene message is transcribed in fertile male testes and may be related to human spermatogenesis.
Zhang S, Qiu W, Wu H, Zhang G, Huang M, Xiao C, Yang J, Kamp C, Huang X, Huellen K, Yue Y, Pan A, Lebo R, Milunsky A, Vogt PH.
Biochem J 2001 Nov; 359(Pt 3):721.
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The shorter zinc finger protein ZNF230 gene message is transcribed in fertile male testes and may be related to human spermatogenesis.
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