TRIM35 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TRIM35.
Immunogen
A synthetic peptide corresponding to N-terminus of human TRIM35.
Sequence
ERSPDVSPGPSRS-C
Host
Goat
Theoretical MW (kDa)
56.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
TRIM35 polyclonal antibody (1 ug/mL) staining of K-562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TRIM35
Entrez GeneID
23087Protein Accession#
NP_741983.2Gene Name
TRIM35
Gene Alias
HLS5, KIAA1098, MAIR, MGC17233
Gene Description
tripartite motif-containing 35
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. [provided by RefSeq
Other Designations
OTTHUMP00000128221
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Interactome
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Publication Reference
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E3 ligase Trim35 inhibits LSD1 demethylase activity through K63-linked ubiquitination and enhances anti-tumor immunity in NSCLC.
Feiyu Tang, Can Lu, Xiang He, Wei Lin, Bowen Xie, Xing Gao, Yang Peng, Desong Yang, Lunquan Sun, Liang Weng.
Cell Reports 2023 Dec; 42(12):113477.
Application:WB, Human, 293FT cells.
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Cloning and characterization of a novel RING-B-box-coiled-coil protein with apoptotic function.
Kimura F, Suzu S, Nakamura Y, Nakata Y, Yamada M, Kuwada N, Matsumura T, Yamashita T, Ikeda T, Sato K, Motoyoshi K.
The Journal of Biological Chemistry 2003 Jul; 278(27):25046.
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E3 ligase Trim35 inhibits LSD1 demethylase activity through K63-linked ubiquitination and enhances anti-tumor immunity in NSCLC.
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