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Last updated: 2016/12/4
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NUP50 polyclonal antibody

  • Catalog # : PAB6369
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of NUP50.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human NUP50.
  • Sequence:
  • C-ELHKILLEKKDA
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 50.1, 46.9
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognise both reported isoforms (NP_009103.2; NP_705931.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Western Blot (0.2-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-3.8 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • NUP50 polyclonal antibody (Cat # PAB6369) staining (0.5 ug/mL) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • NUP50 polyclonal antibody (Cat # PAB6369) (3.8 ug/mL) staining of paraffin embedded human uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_00910.3;NP_705931;NP_710151
  • Gene Name:
  • NUP50
  • Gene Alias:
  • MGC39961,NPAP60,NPAP60L
  • Gene Description:
  • nucleoporin 50kDa
  • Gene Summary:
  • The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000028685,nuclear pore-associated protein 60L
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