NUP50 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
NUP50 polyclonal antibody (Cat # PAB6369) staining (0.5 ug/mL) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NUP50 polyclonal antibody (Cat # PAB6369) (3.8 ug/mL) staining of paraffin embedded human uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NUP50.
Immunogen
A synthetic peptide corresponding to C-terminus of human NUP50.
Sequence
C-ELHKILLEKKDA
Host
Goat
Theoretical MW (kDa)
50.1, 46.9
Reactivity
Human
Specificity
This antibody is expected to recognise both reported isoforms (NP_009103.2; NP_705931.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.2-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-3.8 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
NUP50 polyclonal antibody (Cat # PAB6369) staining (0.5 ug/mL) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NUP50 polyclonal antibody (Cat # PAB6369) (3.8 ug/mL) staining of paraffin embedded human uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NUP50
Entrez GeneID
10762Protein Accession#
NP_00910.3;NP_705931;NP_710151Gene Name
NUP50
Gene Alias
MGC39961, NPAP60, NPAP60L
Gene Description
nucleoporin 50kDa
Omim ID
604646Gene Ontology
HyperlinkGene Summary
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000028685|nuclear pore-associated protein 60L
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Interactome
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Disease
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Publication Reference
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A mechanism of AP-1 suppression through interaction of c-Fos with lamin A/C.
Ivorra C, Kubicek M, Gonzalez JM, Sanz-Gonzalez SM, Alvarez-Barrientos A, O'Connor JE, Burke B, Andres V.
Genes & Development 2006 Feb; 20(3):307.
Application：WB-Tr, Monkey, COS-7 cells.
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A mechanism of AP-1 suppression through interaction of c-Fos with lamin A/C.
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