SNX15 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
SNX15 polyclonal antibody (Cat # PAB6367) staining (0.5 ug/mL) of 293 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SNX15.
Immunogen
A synthetic peptide corresponding to human SNX15.
Sequence
C-EEILRLHLSQLPP
Host
Goat
Theoretical MW (kDa)
38.2, 29.2
Reactivity
Human
Specificity
This antibody is expected to recognize both human isoforms according to NP_037438.2 and NP_680086.2.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (0.2-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SNX15 polyclonal antibody (Cat # PAB6367) staining (0.5 ug/mL) of 293 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SNX15
Entrez GeneID
29907Protein Accession#
NP_037438.2;NP_680086.2Gene Name
SNX15
Gene Alias
HSAF001435
Gene Description
sorting nexin 15
Omim ID
605964Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. Overexpression of this gene results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits. This decrease is caused by the mislocalization of furin, the endoprotease responsible for cleavage of insulin and hepatocyte growth factor receptors. This protein is involved in endosomal trafficking from the plasma membrane to recycling endosomes or the trans-Golgi network. This gene encodes two transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000035515|clone iota unknown protein
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Interactome
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Publication Reference
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Identification and characterization of SNX15, a novel sorting nexin involved in protein trafficking.
Phillips SA, Barr VA, Haft DH, Taylor SI, Haft CR.
The Journal of Biological Chemistry 2001 Feb; 276(7):5074.
Application：IF, WB, Monkey, COS-7 cells.
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Identification and characterization of SNX15, a novel sorting nexin involved in protein trafficking.
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