TRIM69 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
TRIM69 polyclonal antibody (Cat # PAB6364)(0.5 ug/mL) staining of mouse testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TRIM69 polyclonal antibody (Cat # PAB6364) (3.8 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TRIM69.
Immunogen
A synthetic peptide corresponding to C-terminus of human TRIM69.
Sequence
C-GENKEPLHILHPQ
Host
Goat
Theoretical MW (kDa)
57.3
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-3.8 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
TRIM69 polyclonal antibody (Cat # PAB6364)(0.5 ug/mL) staining of mouse testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TRIM69 polyclonal antibody (Cat # PAB6364) (3.8 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TRIM69
Entrez GeneID
140691Protein Accession#
NP_892030.3;NP_542783.2Gene Name
TRIM69
Gene Alias
HSD34, RNF36, Trif
Gene Description
tripartite motif-containing 69
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
ring finger protein 36|trimless|tripartite motif protein 69
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Interactome
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Disease
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Publication Reference
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A novel member of the RBCC family, Trif, expressed specifically in the spermatids of mouse testis.
Shyu HW, Hsu SH, Hsieh-Li HM, Li H.
Mechanisms of Development 2001 Oct; 108(1-2):213.
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A novel member of the RBCC family, Trif, expressed specifically in the spermatids of mouse testis.
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