RNF34 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RNF34.
Immunogen
A synthetic peptide corresponding to N-terminus of human RNF34.
Sequence
KAGATSMWASCC
Host
Goat
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-8 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
RNF34 polyclonal antibody (Cat # PAB6360)(4 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RNF34
Entrez GeneID
80196Protein Accession#
NP_919247.1;NP_079402.2Gene Name
RNF34
Gene Alias
FLJ21786, RFI, RIF, RIFF
Gene Description
ring finger protein 34
Omim ID
608299Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
FYVE-RING finger protein MOMO|RING finger protein RIFF
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Interactome
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Publication Reference
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Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer.
Sasaki S, Nakamura T, Arakawa H, Mori M, Watanabe T, Nagawa H, Croce CM.
Oncogene 2002 Jul; 21(32):5024.
Application:IF, WB-Tr, Human, HeLa, Jurkat cells.
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Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer.
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