ANP32B polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ANP32B.
Immunogen
A synthetic peptide corresponding to human ANP32B.
Sequence
C-KRKRETDDEGEDD
Host
Goat
Theoretical MW (kDa)
28.8
Reactivity
Human
Specificity
This antibody does NOT recognize theTNF family member also known as APRIL - Gene ID number 8741.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(3-5 ug/mL)
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ANP32B polyclonal antibody (Cat # PAB6358) staining (1ug/ml) of human lymph node lysate (RIPA buffer, 30ug total protein per lane) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast with ANP32B polyclonal antibody (Cat # PAB6358) (3.8 ug/mL). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ANP32B
Entrez GeneID
10541Protein Accession#
NP_006392.1Gene Name
ANP32B
Gene Alias
APRIL, PHAPI2, SSP29
Gene Description
acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000021759|OTTHUMP00000063770|acidic protein rich in leucines
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Interactome
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Publication Reference
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Cloning and characterization of a new silver-stainable protein SSP29, a member of the LRR family.
Zhu L, Perlaky L, Henning D, Valdez BC.
Biochemistry and Molecular Biology International 1997 Aug; 42(5):927.
Application:IF, WB-Ce, Human, HeLa, MCF-7 cells.
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Cloning and characterization of a new silver-stainable protein SSP29, a member of the LRR family.
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