CLPP polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
CLPP polyclonal antibody (Cat # PAB6350) staining (1 ug/mL) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CLPP polyclonal antibody (Cat # PAB6350, 2.5 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CLPP.
Immunogen
A synthetic peptide corresponding to human CLPP.
Sequence
C-EAAPAAEPVPAST
Host
Goat
Theoretical MW (kDa)
30.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CLPP polyclonal antibody (Cat # PAB6350) staining (1 ug/mL) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CLPP polyclonal antibody (Cat # PAB6350, 2.5 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CLPP
Entrez GeneID
8192Protein Accession#
NP_006003Gene Name
CLPP
Gene Alias
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Gene Description
ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
Omim ID
601119Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq
Other Designations
ATP-dependent protease ClpAP, proteolytic subunit, human|ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog|endopeptidase Clp
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Interactome
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Publication Reference
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Human ClpP protease: cDNA sequence, tissue-specific expression and chromosomal assignment of the gene.
Bross P, Andresen BS, Knudsen I, Kruse TA, Gregersen N.
FEBS Letters 1995 Dec; 377(2):249.
Application：WB-Ce, WB-Ti, Human, Mammalian cells, Tissues.
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Human ClpP protease: cDNA sequence, tissue-specific expression and chromosomal assignment of the gene.
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