NIF3L1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
NIF3L1 polyclonal antibody (Cat # PAB6347) staining (1ug/ml) of human heart lysate (RIPA buffer, 30ug total protein per lane) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NIF3L1 polyclonal antibody (Cat # PAB6347, 2.5 ug/mL) staining of paraffin embedded human breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NIF3L1.
Immunogen
A synthetic peptide corresponding to human NIF3L1.
Sequence
C-ILSETDRDPLQVV
Host
Goat
Theoretical MW (kDa)
42, 39
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (0.2-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NIF3L1 polyclonal antibody (Cat # PAB6347) staining (1ug/ml) of human heart lysate (RIPA buffer, 30ug total protein per lane) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NIF3L1 polyclonal antibody (Cat # PAB6347, 2.5 ug/mL) staining of paraffin embedded human breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NIF3L1
Entrez GeneID
60491Protein Accession#
NP_068596Gene Name
NIF3L1
Gene Alias
ALS2CR1, CALS-7, MDS015
Gene Description
NIF3 NGG1 interacting factor 3-like 1 (S. pombe)
Omim ID
605778Gene Ontology
HyperlinkGene Summary
S.pombe homolog)-like 1|NIF3 NGG1 interacting factor 3-like 1|amyotrophic lateral sclerosis 2 (juvenile) chromosome region
Other Designations
NIF3 (Ngg1 interacting factor 3, S.pombe homolog)-like 1|NIF3 NGG1 interacting factor 3-like 1|amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 1
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Interactome
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Publication Reference
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Cloning and characterization of three novel genes, ALS2CR1, ALS2CR2, and ALS2CR3, in the juvenile amyotrophic lateral sclerosis (ALS2) critical region at chromosome 2q33-q34: candidate genes for ALS2.
Hadano S, Yanagisawa Y, Skaug J, Fichter K, Nasir J, Martindale D, Koop BF, Scherer SW, Nicholson DW, Rouleau GA, Ikeda J, Hayden MR.
Genomics 2001 Jan; 71(2):200.
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Cloning and characterization of three novel genes, ALS2CR1, ALS2CR2, and ALS2CR3, in the juvenile amyotrophic lateral sclerosis (ALS2) critical region at chromosome 2q33-q34: candidate genes for ALS2.
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