PSMF1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
PSMF1 polyclonal antibody (Cat # PAB6339) staining (1 ug/mL) of human kidney lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing PSMF1 and probed with PSMF1 polyclonal antibody (Cat # PAB6339) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PSMF1.
Immunogen
A synthetic peptide corresponding to human PSMF1.
Sequence
C-DHLPPPGYDDMYL
Host
Goat
Theoretical MW (kDa)
29.8
Reactivity
Human
Specificity
This antibody is expected to recognize isoform 1 (NP_006805.1 and NP_848693.1) only, not isoform 2 (NP_848694.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PSMF1 polyclonal antibody (Cat # PAB6339) staining (1 ug/mL) of human kidney lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing PSMF1 and probed with PSMF1 polyclonal antibody (Cat # PAB6339) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PSMF1
Entrez GeneID
9491Protein Accession#
NP_006805.1;NP_848693.1Gene Name
PSMF1
Gene Alias
PI31
Gene Description
proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
Gene Ontology
HyperlinkGene Summary
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene. [provided by RefSeq
Other Designations
proteasome inhibitor hP131 subunit|proteasome inhibitor subunit 1
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Interactome
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Pathway
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Publication Reference
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cDNA cloning, expression, and functional characterization of PI31, a proline-rich inhibitor of the proteasome.
McCutchen-Maloney SL, Matsuda K, Shimbara N, Binns DD, Tanaka K, Slaughter CA, DeMartino GN.
The Journal of Biological Chemistry 2000 Jun; 275(24):18557.
Application：WB-Re, Recombinant protein.
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cDNA cloning, expression, and functional characterization of PI31, a proline-rich inhibitor of the proteasome.
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