RNF8 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
RNF8 polyclonal antibody (Cat # PAB6337) staining (0.1 ug/mL) of human lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RNF8.
Immunogen
A synthetic peptide corresponding to human RNF8.
Sequence
GEPGFFVTGDRAG-C
Host
Goat
Theoretical MW (kDa)
55.5, 50.8
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_003949.1 and NP_898901.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
RNF8 polyclonal antibody (Cat # PAB6337) staining (0.1 ug/mL) of human lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RNF8
Entrez GeneID
9025Protein Accession#
NP_003949.1;NP_898901.1Gene Name
RNF8
Gene Alias
FLJ12013, KIAA0646
Gene Description
ring finger protein 8
Omim ID
611685Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
C3HC4-type zinc finger protein|OTTHUMP00000016323|OTTHUMP00000039684|UBC13/UEV-interacting ring finger protein|ring finger protein (C3HC4 type) 8
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Interactome
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Disease
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Publication Reference
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Isolation, tissue expression, and chromosomal assignment of a novel human gene which encodes a protein with RING finger motif.
Seki N, Hattori A, Sugano S, Suzuki Y, Nakagawara A, Ohhira M, Muramatsu M, Hori T, Saito T.
Journal of Human Genetics 1998 Dec; 43(4):272.
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Isolation, tissue expression, and chromosomal assignment of a novel human gene which encodes a protein with RING finger motif.
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