RNF2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
RNF2 polyclonal antibody (Cat # PAB6331) staining (1 ug/mL) of human bone marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RNF2.
Immunogen
A synthetic peptide corresponding to human RNF2.
Sequence
C-PMELYYAPTKEHK
Host
Goat
Theoretical MW (kDa)
37.7
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
RNF2 polyclonal antibody (Cat # PAB6331) staining (1 ug/mL) of human bone marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RNF2
Entrez GeneID
6045Protein Accession#
NP_009143.1Gene Name
RNF2
Gene Alias
BAP-1, BAP1, DING, HIPI3, RING1B, RING2
Gene Description
ring finger protein 2
Omim ID
608985Gene Ontology
HyperlinkGene Summary
Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq
Other Designations
OTTHUMP00000033405|OTTHUMP00000060668
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Interactome
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Disease
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Publication Reference
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Binding of the RING polycomb proteins to specific target genes in complex with the grainyhead-like family of developmental transcription factors.
Tuckfield A, Clouston DR, Wilanowski TM, Zhao LL, Cunningham JM, Jane SM.
Molecular and Cellular Biology 2002 Mar; 22(6):1936.
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Binding of the RING polycomb proteins to specific target genes in complex with the grainyhead-like family of developmental transcription factors.
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