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Last updated: 2017/10/15
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MID2 polyclonal antibody

  • Catalog # : PAB6327
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of MID2.
  • Immunogen:
  • A synthetic peptide corresponding to human MID2.
  • Sequence:
  • QESPYVSGMKTCH
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 83.2, 79.9
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody is expected to recognize both human isoforms of this protein.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:32000)
    Western blot (1-3 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • MID2 polyclonal antibody (Cat # PAB6327) staining (1 ug/mL) of mouse heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • MID2 polyclonal antibody (Cat # PAB6327, 5 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_0.36348.1;NP_438112.1
  • Gene Name:
  • MID2
  • Gene Alias:
  • FXY2,MID1,RNF60,TRIM1
  • Gene Description:
  • midline 2
  • Gene Summary:
  • The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000023817,dJ820B18.2 (midline 1 (Opitz/BBB syndrome)),midin 2,tripartite motif protein 1
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