MID2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
MID2 polyclonal antibody (Cat # PAB6327) staining (1 ug/mL) of mouse heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
MID2 polyclonal antibody (Cat # PAB6327, 5 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MID2.
Immunogen
A synthetic peptide corresponding to human MID2.
Sequence
QESPYVSGMKTCH
Host
Goat
Theoretical MW (kDa)
83.2, 79.9
Reactivity
Human, Mouse
Specificity
This antibody is expected to recognize both human isoforms of this protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MID2 polyclonal antibody (Cat # PAB6327) staining (1 ug/mL) of mouse heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
MID2 polyclonal antibody (Cat # PAB6327, 5 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MID2
Entrez GeneID
11043Protein Accession#
NP_0.36348.1;NP_438112.1Gene Name
MID2
Gene Alias
FXY2, MID1, RNF60, TRIM1
Gene Description
midline 2
Omim ID
300204Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000023817|dJ820B18.2 (midline 1 (Opitz/BBB syndrome))|midin 2|tripartite motif protein 1
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Interactome
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Publication Reference
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MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development.
Buchner G, Montini E, Andolfi G, Quaderi N, Cainarca S, Messali S, Bassi MT, Ballabio A, Meroni G, Franco B.
Human Molecular Genetics 1999 Aug; 8(8):1397.
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MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development.
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