CHFR polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CHFR.
Immunogen
A synthetic peptide corresponding to human CHFR.
Sequence
KFNHICEQTRFKN
Host
Goat
Theoretical MW (kDa)
73.4, 73.3, 72.0, 69
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Immunohistochemistry (2.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CHFR polyclonal antibody (Cat # PAB6325) staining (2.5 ug/mL) of paraffin embedded human brain (cerebellum). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CHFR
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Interactome
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Disease
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Publication Reference
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CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation.
Kashima L, Idogawa M, Mita H, Shitashige M, Yamada T, Ogi K, Suzuki H, Toyota M, Ariga H, Sasaki Y, Tokino T.
The Journal of Biological Chemistry 2012 Apr; 287(16):12975.
Application:IP, WB-Tr, Human, HCT-116, HSC-44, MKN45 cells.
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Chfr defines a mitotic stress checkpoint that delays entry into metaphase.
Scolnick DM, Halazonetis TD.
Nature 2000 Jul; 406(6794):430.
Application:WB-Ce, WB-Tr, Human, DLD-1, HCT-116, HT-29, IMR5, NPG, SAOS2, U2OS, SW480 cells.
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CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation.
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