UBE2L3 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of UBE2L3.
Immunogen
A synthetic peptide corresponding to human UBE2L3.
Sequence
C-EFTKKYGEKRPVD
Host
Goat
Theoretical MW (kDa)
17.9
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
UBE2L3 polyclonal antibody (Cat # PAB6308) staining (0.5 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — UBE2L3
Entrez GeneID
7332Protein Accession#
NP_003338Gene Name
UBE2L3
Gene Alias
E2-F1, L-UBC, UBCH7, UbcM4
Gene Description
ubiquitin-conjugating enzyme E2L 3
Omim ID
603721Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
ubiquitin carrier protein|ubiquitin-conjugating enzyme UBCH7|ubiquitin-protein ligase
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Interactome
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Pathway
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Disease
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Publication Reference
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Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5.
Nuber U, Schwarz S, Kaiser P, Schneider R, Scheffner M.
The Journal of Biological Chemistry 1996 Feb; 271(5):2795.
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Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5.
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