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Last updated: 2017/1/15
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UBE2K polyclonal antibody

  • Catalog # : PAB6301
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of UBE2K.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human UBE2K.
  • Sequence:
  • C-SWDVETATELLLSN
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 22.4
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:2000)
    Western Blot (1-3 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • UBE2K polyclonal antibody (Cat # PAB6301) staining (1.5 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • UBE2K polyclonal antibody (Cat # PAB6301)(3.8 ug/mL) staining of paraffin embedded human cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3093
  • Protein Accession#:
  • NP_005330.1;NP_001104582.1;NP_001104583.1
  • Gene Name:
  • UBE2K
  • Gene Alias:
  • DKFZp564C1216,DKFZp686J24237,E2-25K,HIP2,HYPG,LIG
  • Gene Description:
  • ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
  • Gene Summary:
  • The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • huntingtin interacting protein 2,ubiquitin carrier protein,ubiquitin-conjugating enzyme E2-25 KDA,ubiquitin-conjugating enzyme E2-25K,ubiquitin-protein ligase
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