EZH1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
EZH1 polyclonal antibody (Cat # PAB6289) staining (2 ug/mL) of MOLT 4 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of EZH1.
Immunogen
A synthetic peptide corresponding to human EZH1.
Sequence
EIPNPPTSKCITY
Host
Goat
Theoretical MW (kDa)
85.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western blot (2-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
EZH1 polyclonal antibody (Cat # PAB6289) staining (2 ug/mL) of MOLT 4 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EZH1
Entrez GeneID
2145Protein Accession#
NP_001982.2Gene Name
EZH1
Gene Alias
KIAA0388
Gene Description
enhancer of zeste homolog 1 (Drosophila)
Omim ID
601674Gene Ontology
HyperlinkGene Summary
EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM
Other Designations
enhancer of zeste homolog 1
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Interactome
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Disease
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Publication Reference
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Characterization of EZH1, a human homolog of Drosophila Enhancer of zeste near BRCA1.
Abel KJ, Brody LC, Valdes JM, Erdos MR, McKinley DR, Castilla LH, Merajver SD, Couch FJ, Friedman LS, Ostermeyer EA, Lynch ED, King MC, Welcsh PL, Osborne-Lawrence S, Spillman M, Bowcock AM, Collins FS, Weber BL.
Genomics 1996 Oct; 37(2):161.
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Characterization of EZH1, a human homolog of Drosophila Enhancer of zeste near BRCA1.
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