NDRG1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
NDRG1 polyclonal antibody (Cat # PAB6284) staining (0.1 ug/mL) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NDRG1 polyclonal antibody (Cat # PAB6284) (0.5 ug/mL) staining of paraffin embedded human hepatocelluar carcinoma. Data obtained from C. Furer, Visceral Surgery, University of Bern, Switzerland.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NDRG1.
Immunogen
A synthetic peptide corresponding to C-terminus of human NDRG1.
Sequence
GNSAGPKSMEVSC
Host
Goat
Theoretical MW (kDa)
42.8
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NDRG1 polyclonal antibody (Cat # PAB6284) staining (0.1 ug/mL) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NDRG1 polyclonal antibody (Cat # PAB6284) (0.5 ug/mL) staining of paraffin embedded human hepatocelluar carcinoma. Data obtained from C. Furer, Visceral Surgery, University of Bern, Switzerland.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NDRG1
Entrez GeneID
10397Protein Accession#
NP_006087.2Gene Name
NDRG1
Gene Alias
CAP43, CMT4D, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5
Gene Description
N-myc downstream regulated 1
Gene Ontology
HyperlinkGene Summary
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. It is necessary for p53-mediated caspase activation and apoptosis. Mutation in this gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
differentiation-related gene 1 protein|nickel-specific induction protein Cap43|protein regulated by oxygen-1|reducing agents and tunicamycin-responsive protein|tunicamycin-responsive protein
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Interactome
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Disease
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Publication Reference
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Cap43, a novel gene specifically induced by Ni2+ compounds.
Zhou D, Salnikow K, Costa M.
Cancer Research 1998 May; 58(10):2182.
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Cap43, a novel gene specifically induced by Ni2+ compounds.
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