BRF2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
BRF2 polyclonal antibody (Cat # PAB6262) staining (1.5 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of BRF2.
Immunogen
A synthetic peptide corresponding to human BRF2.
Sequence
C-QAARQAATSVPNPP
Host
Goat
Theoretical MW (kDa)
46.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
BRF2 polyclonal antibody (Cat # PAB6262) staining (1.5 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — BRF2
Entrez GeneID
55290Protein Accession#
NP_060780Gene Name
BRF2
Gene Alias
BRFU, FLJ11052, TFIIIB50
Gene Description
BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like
Omim ID
607013Gene Ontology
HyperlinkGene Summary
This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq
Other Designations
RNA polymerase III transcription initiation factor BRF2|RNA polymerase III transcription initiation factor BRFU|transcription factor IIB- related factor, TFIIIB50
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Interactome
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Publication Reference
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Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters.
Schramm L, Pendergrast PS, Sun Y, Hernandez N.
Genes & Development 2000 Oct; 14(20):2650.
Application：WB, Human, HeLa cells, Recombinant protein.
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Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters.
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