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Last updated: 2016/12/4
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GOLM1 polyclonal antibody

  • Catalog # : PAB6261
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of GOLM1.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human GOLM1.
  • Sequence:
  • C-NLLDQREKRNHTL
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 45.3
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:16000)
    Western Blot (0.2-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • GOLM1 polyclonal antibody (Cat # PAB6261)(3.8 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human GOLM1 with C-terminal MYC tag probed with GOLM1 polyclonal antibody (1 ug/mL) in Lane 1 and probed with anti-MYC Tag (1/1000) in lane 3. Mock-transfected HEK293 probed with GOLM1 polyclonal antibody (1mg/mL) in Lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_057632.2;NP_808800.1NP_057632
  • Gene Name:
  • GOLM1
  • Gene Alias:
  • C9orf155,FLJ22634,FLJ23608,GOLPH2,GP73,PSEC0257,bA379P1.3
  • Gene Description:
  • golgi membrane protein 1
  • Gene Summary:
  • The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000021587,golgi membrane protein GP73,golgi phosphoprotein 2,golgi protein, 73-kD
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