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Last updated: 2017/7/23
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DCTN1 polyclonal antibody

  • Catalog # : PAB6249
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of DCTN1.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human DCTN1.
  • Sequence:
  • C-QEQLHQLHSRLIS
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 142, 127, 139, 127
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody is expected to recognise all reported isoforms (NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:128000)
    Western Blot (0.5-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • DCTN1 polyclonal antibody (Cat # PAB6249) staining (1 ug/mL) of human testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • DCTN1 polyclonal antibody (Cat # PAB6249) (2.5 ug/mL) staining staining of paraffin embedded human cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1639
  • Protein Accession#:
  • NP_004073.2;NP_075408.1;NP_001128512.1;NP_001128513.1
  • Gene Name:
  • DCTN1
  • Gene Alias:
  • DAP-150,DP-150,HMN7B,P135
  • Gene Description:
  • dynactin 1 (p150, glued homolog, Drosophila)
  • Gene Summary:
  • This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq
  • Other Designations:
  • 150 kDa dynein-associated polypeptide,dynactin 1,p150, Glued homolog,p150-glued
  • Gene Pathway
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