DCTN1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
DCTN1 polyclonal antibody (Cat # PAB6249) staining (1 ug/mL) of human testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DCTN1 polyclonal antibody (Cat # PAB6249) (2.5 ug/mL) staining staining of paraffin embedded human cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DCTN1.
Immunogen
A synthetic peptide corresponding to C-terminus of human DCTN1.
Sequence
C-QEQLHQLHSRLIS
Host
Goat
Theoretical MW (kDa)
142, 127, 139, 127
Reactivity
Human, Mouse
Specificity
This antibody is expected to recognise all reported isoforms (NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.5-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
DCTN1 polyclonal antibody (Cat # PAB6249) staining (1 ug/mL) of human testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DCTN1 polyclonal antibody (Cat # PAB6249) (2.5 ug/mL) staining staining of paraffin embedded human cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DCTN1
Entrez GeneID
1639Protein Accession#
NP_004073.2;NP_075408.1;NP_001128512.1;NP_001128513.1Gene Name
DCTN1
Gene Alias
DAP-150, DP-150, HMN7B, P135
Gene Description
dynactin 1 (p150, glued homolog, Drosophila)
Gene Ontology
HyperlinkGene Summary
This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq
Other Designations
150 kDa dynein-associated polypeptide|dynactin 1|p150, Glued homolog|p150-glued
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Interactome
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Disease
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Publication Reference
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Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization.
Holzbaur EL, Tokito MK.
Genomics 1996 Feb; 31(3):398.
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Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization.
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