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Last updated: 2022/5/22
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ARL6IP5 polyclonal antibody 

  • Catalog # : PAB6244
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of ARL6IP5.
  • Immunogen:
  • A synthetic peptide corresponding to human ARL6IP5.
  • Sequence:
  • C-NRLTDYISKVKE
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 21.6
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:32000)
    Flow Cytometry (10 ug/mL)
    Immunofluorescence (10 ug/mL)
    Immunohistochemistry (3.75 ug/mL)
    Western Blot (0.3-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • ARL6IP5 polyclonal antibody (Cat # PAB6244) (1 ug/mL) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • ARL6IP5 polyclonal antibody (Cat # PAB6244) (1 ug/mL) staining of K562 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • ARL6IP5 polyclonal antibody (Cat # PAB6244) (3.75 ug/mL) staining of paraffin embedded Human Tonsil (left) and Human Small Intestine (right). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • Immunofluorescence
  • Immunofluorescence
  • ARL6IP5 polyclonal antibody (Cat # PAB6244) Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane and ER/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • ARL6IP5 polyclonal antibody (Cat # PAB6244) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Gene Name:
  • ARL6IP5
  • Gene Alias:
  • DERP11,GTRAP3-18,HSPC127,JWA,PRAF3,addicsin,hp22,jmx
  • Gene Description:
  • ADP-ribosylation-like factor 6 interacting protein 5
  • Gene Summary:
  • Expression of this gene is affected by vitamin A. The encoded protein of this gene may be associated with the cytoskeleton. A similar protein in rats may play a role in the regulation of cell differentiation. The rat protein binds and inhibits the cell membrane glutamate transporter EAAC1. The expression of the rat gene is upregulated by retinoic acid, which results in a specific reduction in EAAC1-mediated glutamate transport. [provided by RefSeq
  • Other Designations:
  • PRA1 domain family 3,cytoskeleton related vitamin A responsive protein,dermal papilla derived protein 11,glutamate transporter EEAC1-associated protein,putative MAPK activating protein PM27
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