ARL6IP5 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
ARL6IP5 polyclonal antibody (Cat # PAB6244) (1 ug/mL) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot (Cell lysate)
ARL6IP5 polyclonal antibody (Cat # PAB6244) (1 ug/mL) staining of K562 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ARL6IP5 polyclonal antibody (Cat # PAB6244) (3.75 ug/mL) staining of paraffin embedded Human Tonsil (top) and Human Small Intestine (bottom). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunofluorescence
ARL6IP5 polyclonal antibody (Cat # PAB6244) Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane and ER/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry
ARL6IP5 polyclonal antibody (Cat # PAB6244) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ARL6IP5.
Immunogen
A synthetic peptide corresponding to human ARL6IP5.
Sequence
C-NRLTDYISKVKE
Host
Goat
Theoretical MW (kDa)
21.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Flow Cytometry (10 ug/mL)
Immunofluorescence (10 ug/mL)
Immunohistochemistry (3.75 ug/mL)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
ARL6IP5 polyclonal antibody (Cat # PAB6244) (1 ug/mL) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Western Blot (Cell lysate)
ARL6IP5 polyclonal antibody (Cat # PAB6244) (1 ug/mL) staining of K562 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ARL6IP5 polyclonal antibody (Cat # PAB6244) (3.75 ug/mL) staining of paraffin embedded Human Tonsil (top) and Human Small Intestine (bottom). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunofluorescence
ARL6IP5 polyclonal antibody (Cat # PAB6244) Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane and ER/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
ARL6IP5 polyclonal antibody (Cat # PAB6244) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — ARL6IP5
Entrez GeneID
10550Protein Accession#
NP_006398.1Gene Name
ARL6IP5
Gene Alias
DERP11, GTRAP3-18, HSPC127, JWA, PRAF3, addicsin, hp22, jmx
Gene Description
ADP-ribosylation-like factor 6 interacting protein 5
Omim ID
605709Gene Ontology
HyperlinkGene Summary
Expression of this gene is affected by vitamin A. The encoded protein of this gene may be associated with the cytoskeleton. A similar protein in rats may play a role in the regulation of cell differentiation. The rat protein binds and inhibits the cell membrane glutamate transporter EAAC1. The expression of the rat gene is upregulated by retinoic acid, which results in a specific reduction in EAAC1-mediated glutamate transport. [provided by RefSeq
Other Designations
PRA1 domain family 3|cytoskeleton related vitamin A responsive protein|dermal papilla derived protein 11|glutamate transporter EEAC1-associated protein|putative MAPK activating protein PM27
-
Interactome
-
Disease
-
Publication Reference
-
Modulation of the neuronal glutamate transporter EAAC1 by the interacting protein GTRAP3-18.
Lin CI, Orlov I, Ruggiero AM, Dykes-Hoberg M, Lee A, Jackson M, Rothstein JD.
Nature 2001 Mar; 410(6824):84.
Application：IF, IP, WB-Ti, Human, Rat, HEK 293 cells, Rat brains.
-
Modulation of the neuronal glutamate transporter EAAC1 by the interacting protein GTRAP3-18.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com