RPS6KB1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
RPS6KB1 polyclonal antibody (Cat # PAB6243) staining (0.2 ug/mL) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
RPS6KB1 polyclonal antibody (Cat # PAB6243)(2 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RPS6KB1.
Immunogen
A synthetic peptide corresponding to C-terminus of human RPS6KB1.
Sequence
C-MISKRPEHLRMNL
Host
Goat
Theoretical MW (kDa)
59.1
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.2-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
RPS6KB1 polyclonal antibody (Cat # PAB6243) staining (0.2 ug/mL) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
RPS6KB1 polyclonal antibody (Cat # PAB6243)(2 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RPS6KB1
Entrez GeneID
6198Protein Accession#
NP_0.03152.1Gene Name
RPS6KB1
Gene Alias
PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha
Gene Description
ribosomal protein S6 kinase, 70kDa, polypeptide 1
Omim ID
608938Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq
Other Designations
p70 S6 kinase, alpha 1|p70 S6 kinase, alpha 2|ribosomal protein S6 kinase, 70kD, polypeptide 1|serine/threonine kinase 14 alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini.
Grove JR, Banerjee P, Balasubramanyam A, Coffer PJ, Price DJ, Avruch J, Woodgett JR.
Molecular and Cellular Biology 1991 Nov; 11(11):5541.
Application：WB-Tr, Monkey, COS-7 cells.
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Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini.
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