Product Browser

Last updated: 2016/12/4
  • Related Product Showcase

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

IRF5 polyclonal antibody

  • Catalog # : PAB6227
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of IRF5.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human IRF5.
  • Sequence:
  • C-QGPWPMHPAGMQ
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 57.8
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize all four reported isoforms (NP_002191.1, NP_1160.32.1, NP_001092097.1, NP_001092099.1). Reported variants represent identical protein: NP_001092101.1, NP_001092098.1, NP_001092097.1 Reported variants represent identical protein: NP_1160.32.1, NP_001092100.1
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Western blot (0.1-0.3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • IRF5 polyclonal antibody (Cat # PAB6227) staining (2 ug/mL) of A-549 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • HEK293 overexpressing human IRF5 and probed with IRF5 polyclonal antibody (Cat # PAB6227) at 1 ug/mL (mock transfection in lane 2) .
  • ELISA
  • Application Image
  • ChIP
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3663
  • Protein Accession#:
  • NP_002191.1;NP_1160.32.1;NP_001092097.1;NP_001092099.1
  • Gene Name:
  • IRF5
  • Gene Alias:
  • SLEB10
  • Gene Description:
  • interferon regulatory factor 5
  • Gene Summary:
  • This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist. [provided by RefSeq
  • Other Designations:
  • -
  • RSS
  • YouTube
  • Linkedin
  • Facebook