IRF5 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of IRF5.
Immunogen
A synthetic peptide corresponding to C-terminus of human IRF5.
Sequence
C-QGPWPMHPAGMQ
Host
Goat
Theoretical MW (kDa)
57.8
Reactivity
Human
Specificity
This antibody is expected to recognize all four reported isoforms (NP_002191.1, NP_1160.32.1, NP_001092097.1, NP_001092099.1). Reported variants represent identical protein: NP_001092101.1, NP_001092098.1, NP_001092097.1 Reported variants represent identical protein: NP_1160.32.1, NP_001092100.1
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot (Cell lysate)
IRF5 polyclonal antibody (Cat # PAB6227) staining (2 ug/mL) of A-549 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing human IRF5 and probed with IRF5 polyclonal antibody (Cat # PAB6227) at 1 ug/mL (mock transfection in lane 2) .Enzyme-linked Immunoabsorbent Assay
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Gene Info — IRF5
Entrez GeneID
3663Protein Accession#
NP_002191.1;NP_1160.32.1;NP_001092097.1;NP_001092099.1Gene Name
IRF5
Gene Alias
SLEB10
Gene Description
interferon regulatory factor 5
Omim ID
607218Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Interferon regulatory factor 5 represses expression of the Epstein-Barr virus oncoprotein LMP1: braking of the IRF7/LMP1 regulatory circuit.
Ning S, Huye LE, Pagano JS.
Journal of Virology 2005 Sep; 79(18):11671.
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Interferon regulatory factor 5 represses expression of the Epstein-Barr virus oncoprotein LMP1: braking of the IRF7/LMP1 regulatory circuit.
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