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Last updated: 2017/1/15
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SF3B3 polyclonal antibody

  • Catalog # : PAB6215
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of SF3B3.
  • Immunogen:
  • A synthetic peptide corresponding to human SF3B3.
  • Sequence:
  • C-VSKKLEDIRTRYAF
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 136
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000)
    Western Blot (0.1-0.3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • SF3B3 polyclonal antibody (Cat # PAB6215) staining (0.1 ug/mL) of nuclear lysate from HeLa cells (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • SF3B3
  • Gene Alias:
  • KIAA0017,RSE1,SAP130,SF3b130,STAF130
  • Gene Description:
  • splicing factor 3b, subunit 3, 130kDa
  • Gene Summary:
  • This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq
  • Other Designations:
  • pre-mRNA splicing factor SF3b, 130 kDa subunit,spliceosome-associated protein 130,splicing factor 3b, subunit 3
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