SF3B3 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SF3B3.
Immunogen
A synthetic peptide corresponding to human SF3B3.
Sequence
C-VSKKLEDIRTRYAF
Host
Goat
Theoretical MW (kDa)
136
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
SF3B3 polyclonal antibody (Cat # PAB6215) staining (0.1 ug/mL) of nuclear lysate from HeLa cells (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — SF3B3
Entrez GeneID
23450Protein Accession#
NP_036558.3Gene Name
SF3B3
Gene Alias
KIAA0017, RSE1, SAP130, SF3b130, STAF130
Gene Description
splicing factor 3b, subunit 3, 130kDa
Omim ID
605592Gene Ontology
HyperlinkGene Summary
This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq
Other Designations
pre-mRNA splicing factor SF3b, 130 kDa subunit|spliceosome-associated protein 130|splicing factor 3b, subunit 3
-
Interactome
-
Disease
-
Publication Reference
-
Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155.
Das BK, Xia L, Palandjian L, Gozani O, Chyung Y, Reed R.
Molecular and Cellular Biology 1999 Oct; 19(10):6796.
Application:WB-Ce, Human, HeLa cells.
-
Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com