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Last updated: 2017/7/23
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SF3B4 polyclonal antibody

  • Catalog # : PAB6214
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of SF3B4.
  • Immunogen:
  • A synthetic peptide corresponding to human SF3B4.
  • Sequence:
  • AAGPISERNQDAT-C
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 44.4
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:32000)
    Western Blot (0.1-0.3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • SF3B4 polyclonal antibody (Cat # PAB6214) staining (0.1 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • SF3B4
  • Gene Alias:
  • MGC10828,SAP49,SF3b49
  • Gene Description:
  • splicing factor 3b, subunit 4, 49kDa
  • Gene Summary:
  • This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000014064,pre-mRNA splicing factor SF3b 49 kDa subunit,spliceosomal protein,spliceosome-associated protein (U2 snRNP),splicing factor 3b, subunit 4
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