SF3B4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
SF3B4 polyclonal antibody (Cat # PAB6214) staining (0.1 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SF3B4.
Immunogen
A synthetic peptide corresponding to human SF3B4.
Sequence
AAGPISERNQDAT-C
Host
Goat
Theoretical MW (kDa)
44.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SF3B4 polyclonal antibody (Cat # PAB6214) staining (0.1 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SF3B4
Entrez GeneID
10262Protein Accession#
NP_005841.1Gene Name
SF3B4
Gene Alias
MGC10828, SAP49, SF3b49
Gene Description
splicing factor 3b, subunit 4, 49kDa
Omim ID
605593Gene Ontology
HyperlinkGene Summary
This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq
Other Designations
OTTHUMP00000014064|pre-mRNA splicing factor SF3b 49 kDa subunit|spliceosomal protein|spliceosome-associated protein (U2 snRNP)|splicing factor 3b, subunit 4
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Interactome
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Publication Reference
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The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site.
Champion-Arnaud P, Reed R.
Genes & Development 1994 Aug; 8(16):1974.
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The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site.
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