ELF3 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
ELF3 polyclonal antibody (Cat # PAB6195) staining (3 ug/mL) of NCI-H460 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing ELF3 and probed with ELF3 polyclonal antibody (Cat # PAB6195) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ELF3.
Immunogen
A synthetic peptide corresponding to human ELF3.
Sequence
C-SGWKEEEVLQSRN
Host
Goat
Theoretical MW (kDa)
41.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ELF3 polyclonal antibody (Cat # PAB6195) staining (3 ug/mL) of NCI-H460 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing ELF3 and probed with ELF3 polyclonal antibody (Cat # PAB6195) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ELF3
Entrez GeneID
1999Protein Accession#
NP_004424.2Gene Name
ELF3
Gene Alias
EPR-1, ERT, ESE-1, ESX
Gene Description
E74-like factor 3 (ets domain transcription factor, epithelial-specific )
Omim ID
602191Gene Ontology
HyperlinkGene Summary
O
Other Designations
E74-like factor 3 (ETS domain transcription factor, serine box, epithelial-specific)|E74-like factor 3 (ets domain transcription factor)|OTTHUMP00000034050|OTTHUMP00000034051|epithelial-restricted with serine box|epithelium-restricted Ets protein ESX|ets
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Interactome
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Publication Reference
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Isolation and characterization of a novel epithelium-specific transcription factor, ESE-1, a member of the ets family.
Oettgen P, Alani RM, Barcinski MA, Brown L, Akbarali Y, Boltax J, Kunsch C, Munger K, Libermann TA.
Molecular and Cellular Biology 1997 Aug; 17(8):4419.
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Isolation and characterization of a novel epithelium-specific transcription factor, ESE-1, a member of the ets family.
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