KIF5B polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of KIF5B.
Immunogen
A synthetic peptide corresponding to C-terminus of human KIF5B.
Sequence
C-QPVAVRGGGGKQV
Host
Goat
Theoretical MW (kDa)
110
Reactivity
Dog, Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-1 ug/mL)
Immunohistochemistry (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
KIF5B polyclonal antibody (Cat # PAB6192) staining (0.2 ug/mL) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
KIF5B polyclonal antibody (Cat # PAB6192) staining (1 ug/mL) of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KIF5B
Entrez GeneID
3799Protein Accession#
NP_004512.1Gene Name
KIF5B
Gene Alias
KINH, KNS, KNS1, UKHC
Gene Description
kinesin family member 5B
Omim ID
602809Gene Ontology
HyperlinkOther Designations
kinesin 1 (110-120kD)|kinesin heavy chain
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Interactome
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Disease
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Publication Reference
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Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules.
Semiz S, Park JG, Nicoloro SM, Furcinitti P, Zhang C, Chawla A, Leszyk J, Czech MP.
The EMBO Journal 2003 May; 22(10):2387.
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Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules.
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