KLF8 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of KLF8.
Immunogen
A synthetic peptide corresponding to human KLF8.
Sequence
VDMDKLINNLEVQ-C
Host
Goat
Theoretical MW (kDa)
39.3, 27.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
KLF8 polyclonal antibody (Cat # PAB6183) staining (2 ug/mL) of human kidney lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KLF8
Entrez GeneID
11279Protein Accession#
NP_009181.2Gene Name
KLF8
Gene Alias
BKLF3, DKFZp686O08126, DXS741, MGC138314, ZNF741
Gene Description
Kruppel-like factor 8
Omim ID
300286Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A pseudogene has been identified on chromosome 16. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
zinc finger protein 741
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Interactome
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Disease
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Publication Reference
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Human Kruppel-like factor 8: a CACCC-box binding protein that associates with CtBP and represses transcription.
van Vliet J, Turner J, Crossley M.
Nucleic Acids Research 2000 May; 28(9):1955.
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Human Kruppel-like factor 8: a CACCC-box binding protein that associates with CtBP and represses transcription.
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