FLOT1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FLOT1.
Immunogen
A synthetic peptide corresponding to human FLOT1.
Sequence
C-SISQVNHKPLRTA
Host
Goat
Theoretical MW (kDa)
47.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Immunohistochemistry (5 ug/mL)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
FLOT1 polyclonal antibody (Cat # PAB6176) staining (0.3 ug/mL) of NCI-H460 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing FLOT1 and probed with FLOT1 polyclonal antibody (Cat # PAB6176) (mock transfection in first lane), tested by Origene.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with FLOT1 polyclonal antibody (Cat # PAB6176) (3.8 ug/mL). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FLOT1
Entrez GeneID
10211Protein Accession#
NP_005794Gene Name
FLOT1
Gene Alias
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Gene Description
flotillin 1
Omim ID
606998Gene Ontology
HyperlinkGene Summary
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000029208|integral membrane component of caveolae
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Interactome
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Pathway
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Disease
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Publication Reference
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Acute ethanol treatment modulates Toll-like receptor-4 association with lipid rafts.
Dolganiuc A, Bakis G, Kodys K, Mandrekar P, Szabo G.
Alcoholism, Clinical and Experimental Research 2006 Jan; 30(1):76.
Application:WB-Ce, Mouse, CHO cells.
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Acute ethanol treatment modulates Toll-like receptor-4 association with lipid rafts.
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