GULP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
GULP1 polyclonal antibody (Cat # PAB6174) staining (0.2 ug/mL) of human placenta lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GULP1 polyclonal antibody (Cat # PAB6174, 5 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GULP1.
Immunogen
A synthetic peptide corresponding to human GULP1.
Sequence
EGTVFCLDPLDSRC
Host
Goat
Theoretical MW (kDa)
34.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western blot (0.2-0.6 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GULP1 polyclonal antibody (Cat # PAB6174) staining (0.2 ug/mL) of human placenta lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GULP1 polyclonal antibody (Cat # PAB6174, 5 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GULP1
Entrez GeneID
51454Protein Accession#
NP_057399.1Gene Name
GULP1
Gene Alias
CED-6, CED6, FLJ31156, GULP
Gene Description
GULP, engulfment adaptor PTB domain containing 1
Omim ID
608165Gene Ontology
HyperlinkGene Summary
The prompt clearance of cells undergoing apoptosis is critical during embryonic development, normal tissue turnover, inflammation, and autoimmunity. CED6 is an evolutionarily conserved adaptor protein required for efficient engulfment of apoptotic cells by phagocytes.[supplied by OMIM
Other Designations
PTB domain adaptor protein CED-6|engulfment adapter protein
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Interactome
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Disease
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Publication Reference
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Interaction of CED-6/GULP, an adapter protein involved in engulfment of apoptotic cells with CED-1 and CD91/low density lipoprotein receptor-related protein (LRP).
Su HP, Nakada-Tsukui K, Tosello-Trampont AC, Li Y, Bu G, Henson PM, Ravichandran KS.
The Journal of Biological Chemistry 2002 Apr; 277(14):11772.
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Interaction of CED-6/GULP, an adapter protein involved in engulfment of apoptotic cells with CED-1 and CD91/low density lipoprotein receptor-related protein (LRP).
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