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Last updated: 2016/12/4
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DAP3 polyclonal antibody

  • Catalog # : PAB6163
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of DAP3.
  • Immunogen:
  • A synthetic peptide corresponding to human DAP3.
  • Sequence:
  • NPSLLERHCAYL
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 45.6
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize both human isoforms of this protein.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:16000)
    Western blot (1-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • DAP3 polyclonal antibody (Cat # PAB6163) staining (3 ug/mL) of HeLa lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • HEK293 overexpressing DAP3 and probed with DAP3 polyclonal antibody (Cat # PAB6163) (mock transfection in first lane), tested by Origene.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7818
  • Protein Accession#:
  • NP_387506.1;NP_004623.1
  • Gene Name:
  • DAP3
  • Gene Alias:
  • DAP-3,DKFZp686G12159,MGC126058,MGC126059,MRP-S29,MRPS29,bMRP-10
  • Gene Description:
  • death associated protein 3
  • Gene Summary:
  • Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000033480,OTTHUMP00000033481,death-associated protein 3,mitochondrial 28S ribosomal protein S29
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