DAP3 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
DAP3 polyclonal antibody (Cat # PAB6163) staining (3 ug/mL) of HeLa lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing DAP3 and probed with DAP3 polyclonal antibody (Cat # PAB6163) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DAP3.
Immunogen
A synthetic peptide corresponding to human DAP3.
Sequence
NPSLLERHCAYL
Host
Goat
Theoretical MW (kDa)
45.6
Reactivity
Human
Specificity
This antibody is expected to recognize both human isoforms of this protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
DAP3 polyclonal antibody (Cat # PAB6163) staining (3 ug/mL) of HeLa lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing DAP3 and probed with DAP3 polyclonal antibody (Cat # PAB6163) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DAP3
Entrez GeneID
7818Protein Accession#
NP_387506.1;NP_004623.1Gene Name
DAP3
Gene Alias
DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10
Gene Description
death associated protein 3
Omim ID
602074Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq
Other Designations
OTTHUMP00000033480|OTTHUMP00000033481|death-associated protein 3|mitochondrial 28S ribosomal protein S29
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Interactome
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Disease
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Publication Reference
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Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death.
Kissil JL, Deiss LP, Bayewitch M, Raveh T, Khaspekov G, Kimchi A.
The Journal of Biological Chemistry 1995 Nov; 270(46):27932.
Application：WB-Ce, WB-Re, WB-Tr, Human, HeLa cells, Recombinant proteins.
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Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death.
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