RGS1 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RGS1.
Immunogen
A synthetic peptide corresponding to human RGS1.
Sequence
C-NLLNDLQANSLK
Host
Goat
Theoretical MW (kDa)
23.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
RGS1 polyclonal antibody (Cat # PAB6159) staining (1 ug/mL) of HepG2 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RGS1
Entrez GeneID
5996Protein Accession#
NP_002913Gene Name
RGS1
Gene Alias
1R20, BL34, IER1, IR20
Gene Description
regulator of G-protein signaling 1
Omim ID
600323Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. [provided by RefSeq
Other Designations
B-cell activation protein BL34|OTTHUMP00000033591|early response protein 1R20|immediate-early response 1, B-cell specific|regulator of G-protein signalling 1
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Interactome
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Disease
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