KLF3 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of KLF3.
Immunogen
A synthetic peptide corresponding to human KLF3.
Sequence
LMFDPVPVKQEAMD-C
Host
Goat
Theoretical MW (kDa)
38.8
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
KLF3 polyclonal antibody (Cat # PAB6147) staining (1 ug/mL) of human heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KLF3
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Interactome
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Disease
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Publication Reference
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KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.
Jing Hao, Xi Yang, Chao Zhang, Xue-Tao Zhang, Ming Shi, Shao-Hua Wang, Li Mi, Yu-Ting Zhao, Huiqing Cao, Yangming Wang.
Cell proliferation 2020 Nov; 53(11):e12914.
Application:IF, WB-Ce, WB-Ti, Mouse, Hepa1-6, CT26, mESCs (V6.5), Mouse brain, Mouse embryo, Mouse heart, Mouse liver, Mouse skeletal muscle.
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A Feedback Loop Consisting of MicroRNA 23a/27a and the β-Like Globin Suppressors KLF3 and SP1 Regulates Globin Gene Expression.
Ma Y, Wang B, Jiang F, Wang D, Liu H, Yan Y, Dong H, Wang F, Gong B, Zhu Y, Dong L, Yin H, Zhang Z, Zhao H, Wu Z, Zhang J, Zhou J, Yu J.
Molecular and Cellular Biology 2013 Oct; 33(20):3994.
Application:WB-Tr, ChIP, Human, K562 cells.
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Basic Kruppel-like factor functions within a network of interacting haematopoietic transcription factors.
Turner J, Crossley M.
The International Journal of Biochemistry & Cell Biology 1999 Oct; 31(10):1169.
Application:WB-Ce, WB-Tr, Human, Mammalian cells.
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KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.
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