NQO1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
NQO1 polyclonal antibody (Cat # PAB6134) (0.3 ug/mL) staining of Rat Kidney (1), Lung (2) and Duodenum (3) lysate. (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot (Cell lysate)
NQO1 polyclonal antibody (Cat # PAB6134) (0.3 ug/mL) staining of U251 (1) and HepG2 (2) cell lysate. (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NQO1 polyclonal antibody (Cat # PAB6134) (2 ug/mL) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunofluorescence
NQO1 polyclonal antibody (Cat # PAB6134) Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NQO1.
Immunogen
A synthetic peptide corresponding to C-terminus of human NQO1.
Sequence
C-SIPTDNQIKARK
Host
Goat
Theoretical MW (kDa)
30.9, 27.3, 26.4
Reactivity
Human, Rat
Specificity
This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Immunofluorescence (10 ug/mL)
Immunohistochemistry (2-4 ug/mL)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
NQO1 polyclonal antibody (Cat # PAB6134) (0.3 ug/mL) staining of Rat Kidney (1), Lung (2) and Duodenum (3) lysate. (35 ug protein in RIPA buffer). Detected by chemiluminescence.Western Blot (Cell lysate)
NQO1 polyclonal antibody (Cat # PAB6134) (0.3 ug/mL) staining of U251 (1) and HepG2 (2) cell lysate. (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NQO1 polyclonal antibody (Cat # PAB6134) (2 ug/mL) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunofluorescence
NQO1 polyclonal antibody (Cat # PAB6134) Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
-
Gene Info — NQO1
Entrez GeneID
1728Protein Accession#
NP_000894.1;NP_001020604.1;NP_001020605.1Gene Name
NQO1
Gene Alias
DHQU, DIA4, DTD, NMOR1, NMORI, QR1
Gene Description
NAD(P)H dehydrogenase, quinone 1
Omim ID
125860Gene Ontology
HyperlinkGene Summary
This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
DT-diaphorase|NAD(P)H menadione oxidoreductase 1, dioxin-inducible|NAD(P)H:Quinone acceptor oxidoreductase type 1|NAD(P)H:menadione oxidoreductase 1|NAD(P)H:quinone oxireductase|azoreductase|diaphorase (NADH/NADPH) (cytochrome b-5 reductase)|diaphorase-4|
-
Interactome
-
Disease
-
Publication Reference
-
NQO1 stabilizes p53 through a distinct pathway.
Asher G, Lotem J, Kama R, Sachs L, Shaul Y.
PNAS 2002 Mar; 99(5):3099.
-
NQO1 stabilizes p53 through a distinct pathway.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com