RP6-213H19.1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
RP6-213H19.1 polyclonal antibody (Cat # PAB6133) staining (0.5 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
RP6-213H19.1 polyclonal antibody (Cat # PAB6133) (2.5 ug/mL) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RP6-213H19.1.
Immunogen
A synthetic peptide corresponding to human RP6-213H19.1.
Sequence
KLIEKFQKCSADESP
Host
Goat
Theoretical MW (kDa)
46.5, 37.8, 39.7
Reactivity
Human
Specificity
This antibody is expected to recognize all three reported isoforms (NP_057626.2, NP_001035918.1 and NP_001035917.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Immunohistochemistry (2.5 ug/mL)
Western Blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
RP6-213H19.1 polyclonal antibody (Cat # PAB6133) staining (0.5 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
RP6-213H19.1 polyclonal antibody (Cat # PAB6133) (2.5 ug/mL) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RP6-213H19.1
Entrez GeneID
51765Protein Accession#
NP_057626.2;NP_001035918.1;NP_001035917.1Gene Name
RP6-213H19.1
Gene Alias
MASK, MST4
Gene Description
serine/threonine protein kinase MST4
Omim ID
300547Gene Ontology
HyperlinkGene Summary
The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq
Other Designations
Mst3 and SOK1-related kinase|STE20-like kinase MST4|mammalian Ste20-like protein kinase 4|mammalian sterile 20-like 4|serine/threonine protein kinase MASK
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Interactome
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Publication Reference
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Cloning of MASK, a novel member of the mammalian germinal center kinase III subfamily, with apoptosis-inducing properties.
Dan I, Ong SE, Watanabe NM, Blagoev B, Nielsen MM, Kajikawa E, Kristiansen TZ, Mann M, Pandey A.
The Journal of Biological Chemistry 2002 Feb; 277(8):5929.
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Cloning of MASK, a novel member of the mammalian germinal center kinase III subfamily, with apoptosis-inducing properties.
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