DKK4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
DKK4 polyclonal antibody (Cat # PAB6130) staining (1 ug/mL) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DKK4.
Immunogen
A synthetic peptide corresponding to human DKK4.
Sequence
RQHARLRVCQKIEKL
Host
Goat
Theoretical MW (kDa)
24.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
DKK4 polyclonal antibody (Cat # PAB6130) staining (1 ug/mL) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DKK4
Entrez GeneID
27121Protein Accession#
NP_055235Gene Name
DKK4
Gene Alias
DKK-4, MGC129562, MGC129563
Gene Description
dickkopf homolog 4 (Xenopus laevis)
Omim ID
605417Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. [provided by RefSeq
Other Designations
dickkopf homolog 4
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Interactome
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Pathway
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Disease
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Publication Reference
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Functional and structural diversity of the human Dickkopf gene family.
Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA.
Gene 1999 Oct; 238(2):301.
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Functional and structural diversity of the human Dickkopf gene family.
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