DKK3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
DKK3 polyclonal antibody (Cat # PAB6129) staining (2 ug/mL) of HEK 293T (Bosc 23) lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DKK3 polyclonal antibody (Cat # PAB6129)(5 ug/mL) staining of paraffin embedded human cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DKK3.
Immunogen
A synthetic peptide corresponding to C-terminus of human DKK3.
Sequence
C-AAAALLGGEEI
Host
Goat
Theoretical MW (kDa)
38.4
Reactivity
Human
Specificity
NP_0.37385.2, NP_056965.3 and NP_001018067.1 are varients of the same protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.03-0.1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
DKK3 polyclonal antibody (Cat # PAB6129) staining (2 ug/mL) of HEK 293T (Bosc 23) lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DKK3 polyclonal antibody (Cat # PAB6129)(5 ug/mL) staining of paraffin embedded human cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DKK3
Entrez GeneID
27122Protein Accession#
NP_0.37385.2;NP_056965.3;NP_001018067.1Gene Name
DKK3
Gene Alias
REIC
Gene Description
dickkopf homolog 3 (Xenopus laevis)
Omim ID
605416Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq
Other Designations
RIG-like 5-6|RIG-like 7-1|dickkopf 3|dickkopf homolog 3
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Interactome
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Disease
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Publication Reference
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Functional and structural diversity of the human Dickkopf gene family.
Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA.
Gene 1999 Oct; 238(2):301.
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Functional and structural diversity of the human Dickkopf gene family.
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