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Last updated: 2016/12/11
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DKK3 polyclonal antibody

  • Catalog # : PAB6129
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of DKK3.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human DKK3.
  • Sequence:
  • C-AAAALLGGEEI
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 38.4
  • Reactivity:
  • Human
  • Specificity:
  • NP_0.37385.2, NP_056965.3 and NP_001018067.1 are varients of the same protein.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:16000)
    Western Blot (0.03-0.1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • DKK3 polyclonal antibody (Cat # PAB6129) staining (2 ug/mL) of HEK 293T (Bosc 23) lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • DKK3 polyclonal antibody (Cat # PAB6129)(5 ug/mL) staining of paraffin embedded human cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_0.37385.2;NP_056965.3;NP_001018067.1
  • Gene Name:
  • DKK3
  • Gene Alias:
  • REIC
  • Gene Description:
  • dickkopf homolog 3 (Xenopus laevis)
  • Gene Summary:
  • This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq
  • Other Designations:
  • RIG-like 5-6,RIG-like 7-1,dickkopf 3,dickkopf homolog 3
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