STUB1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of STUB1.
Immunogen
A synthetic peptide corresponding to human STUB1.
Sequence
C-DAFISENGWVEDY
Host
Goat
Theoretical MW (kDa)
34.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
STUB1 polyclonal antibody (Cat # PAB6119) staining (0.3 ug/mL) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — STUB1
Entrez GeneID
10273Protein Accession#
NP_005852.2Gene Name
STUB1
Gene Alias
CHIP, HSPABP2, NY-CO-7, SDCCAG7, UBOX1
Gene Description
STIP1 homology and U-box containing protein 1
Omim ID
607207Gene Ontology
HyperlinkGene Summary
STUB1, or CHIP, is a ubiquitin ligase/cochaperone that participates in protein quality control by targeting a broad range of chaperone protein substrates for degradation (Min et al., 2008 [PubMed 18411298]).[supplied by OMIM
Other Designations
heat shock protein A binding protein 2 (c-terminal)|serologically defined colon cancer antigen 7
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Interactome
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Pathway
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Publication Reference
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CHIP is a U-box-dependent E3 ubiquitin ligase: identification of Hsc70 as a target for ubiquitylation.
Jiang J, Ballinger CA, Wu Y, Dai Q, Cyr DM, Hohfeld J, Patterson C.
The Journal of Biological Chemistry 2001 Nov; 276(46):42938.
Application:WB-Re, WB-Tr, Monkey, COS-7 cells, Recombinant protein.
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CHIP is a U-box-dependent E3 ubiquitin ligase: identification of Hsc70 as a target for ubiquitylation.
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