RACGAP1 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RACGAP1.
Immunogen
A synthetic peptide corresponding to C-terminus of human RACGAP1.
Sequence
C-GRQGNFFASPMLK
Host
Goat
Theoretical MW (kDa)
71
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL))
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
RACGAP1 polyclonal antibody (Cat # PAB6112)(1ug/mL) staining of lysate of cell line K-562 (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
RACGAP1 polyclonal antibody (Cat # PAB6112) staining (1 ug/mL) of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RACGAP1
Entrez GeneID
29127Protein Accession#
NP_037409.2;NP_001119576.1;NP_001119575.1Gene Name
RACGAP1
Gene Alias
HsCYK-4, ID-GAP, MgcRacGAP
Gene Description
Rac GTPase activating protein 1
Omim ID
604980Gene Ontology
HyperlinkGene Summary
Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.[supplied by OMIM
Other Designations
GTPase activating protein
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Interactome
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Disease
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Publication Reference
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Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis.
Nishimura Y, Yonemura S.
Journal of Cell Science 2005 Dec; 119(Pt 1):104.
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Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis.
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