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RACGAP1 polyclonal antibody

  • Catalog # : PAB6112
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of RACGAP1.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human RACGAP1.
  • Sequence:
  • C-GRQGNFFASPMLK
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 71
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Western Blot (1-3 ug/mL))
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • RACGAP1 polyclonal antibody (Cat # PAB6112)(1ug/mL) staining of lysate of cell line K-562 (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • RACGAP1 polyclonal antibody (Cat # PAB6112) staining (1 ug/mL) of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_037409.2;NP_001119576.1;NP_001119575.1
  • Gene Name:
  • RACGAP1
  • Gene Alias:
  • HsCYK-4,ID-GAP,MgcRacGAP
  • Gene Description:
  • Rac GTPase activating protein 1
  • Gene Summary:
  • Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.[supplied by OMIM
  • Other Designations:
  • GTPase activating protein
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