RAC2 polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of RAC2.
Immunogen
A synthetic peptide corresponding to human RAC2.
Sequence
PQPTRQQKRACSLL
Host
Goat
Theoretical MW (kDa)
21.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
RAC2 polyclonal antibody (Cat # PAB6111) staining (0.3 ug/mL) of human tonsil lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
RAC2 polyclonal antibody (Cat # PAB6111) staining (0.3 ug/mL) of paraffin embedded human tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.A) Strong staining in the interfollicular area and mantle zone.B) Positive cells in the interfollicular area.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RAC2
Entrez GeneID
5880Protein Accession#
NP_002863.1Gene Name
RAC2
Gene Alias
EN-7, Gx, HSPC022
Gene Description
ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. [provided by RefSeq
Other Designations
Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2)|ras-related C3 botulinum toxin substrate 2|rho family, small GTP binding protein Rac2|small G protein
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Interactomes
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Pathways
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Diseases
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Publication Reference
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A hemopoietic specific gene encoding a small GTP binding protein is overexpressed during T cell activation.
Reibel L, Dorseuil O, Stancou R, Bertoglio J, Gacon G.
Biochemical and Biophysical Research Communications 1991 Mar; 175(2):451.
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A hemopoietic specific gene encoding a small GTP binding protein is overexpressed during T cell activation.
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