ANXA11 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
ANXA11 polyclonal antibody (Cat # PAB6107) staining (0.2ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ANXA11 polyclonal antibody (Cat # PAB6107, 3.8 ug/mL) staining of paraffin embedded human placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ANXA11.
Immunogen
A synthetic peptide corresponding to human ANXA11.
Sequence
YPGYPPPPGGYPP-C
Host
Goat
Theoretical MW (kDa)
54.4
Reactivity
Human
Specificity
NP_001148.1, NP_665875.1 and NP_665876.1 are varients that encode the same protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (0.2-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ANXA11 polyclonal antibody (Cat # PAB6107) staining (0.2ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ANXA11 polyclonal antibody (Cat # PAB6107, 3.8 ug/mL) staining of paraffin embedded human placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ANXA11
Entrez GeneID
311Protein Accession#
NP_001148.1;NP_665875.1;NP_665876.1Gene Name
ANXA11
Gene Alias
ANX11, CAP50
Gene Description
annexin A11
Omim ID
602572Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain the calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Transcript variants encoding the same isoform have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000019955|OTTHUMP00000019956|OTTHUMP00000019957|OTTHUMP00000019958|OTTHUMP00000059806|annexin XI|autoantigen, 56-kD|calcyclin-associated annexin 50
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Interactome
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Disease
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Publication Reference
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The 56K autoantigen is identical to human annexin XI.
Misaki Y, Pruijn GJ, van der Kemp AW, van Venrooij WJ.
The Journal of Biological Chemistry 1994 Feb; 269(6):4240.
Application：IF, Human, HEp-2 cells.
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The 56K autoantigen is identical to human annexin XI.
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