NUMB polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
NUMB polyclonal antibody (Cat # PAB6102) staining (0.03 ug/mL) of mouse brain lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NUMB.
Immunogen
A synthetic peptide corresponding to C-terminus of human NUMB.
Sequence
C-PFSSDLQKTFEIEL
Host
Goat
Theoretical MW (kDa)
70.8, 65.9, 69.4, 64
Reactivity
Human, Mouse
Specificity
This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_0.03735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NUMB polyclonal antibody (Cat # PAB6102) staining (0.03 ug/mL) of mouse brain lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — NUMB
Entrez GeneID
8650Protein Accession#
NP_001005743.1;001005744.1;NP_0.03735;NP_001005745.1Gene Name
NUMB
Gene Alias
S171
Gene Description
numb homolog (Drosophila)
Omim ID
603728Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
numb homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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Asymmetric localization of numb in the chick somite and the influence of myogenic signals.
Holowacz T, Zeng L, Lassar AB.
Developmental Dynamics 2006 Mar; 235(3):633.
Application：IF, IHC-Fr, Chicken, Chicken embryos.
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G protein betagamma subunits and AGS3 control spindle orientation and asymmetric cell fate of cerebral cortical progenitors.
Sanada K, Tsai LH.
Cell 2005 Jul; 122(1):119.
Application：IF, IHC, Mouse, Mouse cortex.
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Asymmetric localization of numb in the chick somite and the influence of myogenic signals.
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