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Last updated: 2022/7/3
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FOXP3 polyclonal antibody 

  • Catalog # : PAB6100
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of FOXP3.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human FOXP3.
  • Sequence:
  • SQRPSRCSNPTPGP
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 47.2, 43.4
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:8000)
    Flow Cytometry (10 ug/mL)
    Immunofluorescence (10 ug/mL)
    Immunohistochemistry (6-8 ug/mL)
    Western Blot (1-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ChIP
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • FOXP3 polyclonal antibody (Cat # PAB6100) (1 ug/mL) staining of Human Muscle (1), (2 ug/mL) MOLT4 (2) and (1 ug/mL) negative control Pancreas (3) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • FOXP3 polyclonal antibody (Cat # PAB6100) (8 ug/mL) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
  • Immunofluorescence
  • Immunofluorescence
  • FOXP3 polyclonal antibody (Cat # PAB6100) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • FOXP3 polyclonal antibody (Cat # PAB6100) Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • ChIP
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Protein Accession#:
  • NP_054728.2;NP_001107849.1
  • Gene Name:
  • FOXP3
  • Gene Alias:
  • AIID,DIETER,IPEX,JM2,MGC141961,MGC141963,PIDX,XPID
  • Gene Description:
  • forkhead box P3
  • Gene Summary:
  • The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000025832,immune dysregulation, polyendocrinopathy, enteropathy, X-linked,immunodeficiency, polyendocrinopathy, enteropathy, X-linked,scurfin
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