FOXP3 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
FOXP3 polyclonal antibody (Cat # PAB6100) (1 ug/mL) staining of Human Muscle (1), (2 ug/mL) MOLT4 (2) and (1 ug/mL) negative control Pancreas (3) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FOXP3 polyclonal antibody (Cat # PAB6100) (8 ug/mL) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
Immunofluorescence
FOXP3 polyclonal antibody (Cat # PAB6100) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).
Flow Cytometry
FOXP3 polyclonal antibody (Cat # PAB6100) Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FOXP3.
Immunogen
A synthetic peptide corresponding to C-terminus of human FOXP3.
Sequence
SQRPSRCSNPTPGP
Host
Goat
Theoretical MW (kDa)
47.2, 43.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Flow Cytometry (10 ug/mL)
Immunofluorescence (10 ug/mL)
Immunohistochemistry (6-8 ug/mL)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot (Tissue lysate)
FOXP3 polyclonal antibody (Cat # PAB6100) (1 ug/mL) staining of Human Muscle (1), (2 ug/mL) MOLT4 (2) and (1 ug/mL) negative control Pancreas (3) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FOXP3 polyclonal antibody (Cat # PAB6100) (8 ug/mL) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.Immunofluorescence
FOXP3 polyclonal antibody (Cat # PAB6100) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
FOXP3 polyclonal antibody (Cat # PAB6100) Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — FOXP3
Entrez GeneID
50943Protein Accession#
NP_054728.2;NP_001107849.1Gene Name
FOXP3
Gene Alias
AIID, DIETER, IPEX, JM2, MGC141961, MGC141963, PIDX, XPID
Gene Description
forkhead box P3
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000025832|immune dysregulation, polyendocrinopathy, enteropathy, X-linked|immunodeficiency, polyendocrinopathy, enteropathy, X-linked|scurfin
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Interactome
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Disease
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Publication Reference
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The immune paradox of sarcoidosis and regulatory T cells.
Miyara M, Amoura Z, Parizot C, Badoual C, Dorgham K, Trad S, Kambouchner M, Valeyre D, Chapelon-Abric C, Debre P, Piette JC, Gorochov G.
The Journal of Experimental Medicine 2006 Jan; 203(2):359.
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Differentiation and expansion of T cells with regulatory function from human peripheral lymphocytes by stimulation in the presence of TGF-{beta}.
Rao PE, Petrone AL, Ponath PD.
Journal of Immunology 2005 Feb; 174(3):1446.
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The immune paradox of sarcoidosis and regulatory T cells.
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