SIAH1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
SIAH1 polyclonal antibody (Cat # PAB6099) staining (1 ug/mL) of human liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Tissue lysate)
SIAH1 polyclonal antibody (Cat # PAB6099) (1 ug/mL) staining of RatLiver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence
SIAH1 polyclonal antibody (Cat # PAB6099) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing mitochondrial/cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SIAH1.
Immunogen
A synthetic peptide corresponding to N-terminus of human SIAH1.
Sequence
SRQTATALPTGTSKC
Host
Goat
Theoretical MW (kDa)
31.1, 34.6
Reactivity
Human, Rat
Specificity
This antibody is expected to recognize both reported isoforms (according to NP_003022.3; NP_001006611.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Immunofluorescence (10 ug/mL)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SIAH1 polyclonal antibody (Cat # PAB6099) staining (1 ug/mL) of human liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Tissue lysate)
SIAH1 polyclonal antibody (Cat # PAB6099) (1 ug/mL) staining of RatLiver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunofluorescence
SIAH1 polyclonal antibody (Cat # PAB6099) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing mitochondrial/cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
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Gene Info — SIAH1
Entrez GeneID
6477Protein Accession#
NP_003022.3;NP_001006611.1Gene Name
SIAH1
Gene Alias
FLJ08065, HUMSIAH, Siah-1, Siah-1a, hSIAH1
Gene Description
seven in absentia homolog 1 (Drosophila)
Omim ID
602212Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq
Other Designations
seven in absentia homolog 1|sonic hedgehog homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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Siah-1L, a novel transcript variant belonging to the human Siah family of proteins, regulates beta-catenin activity in a p53-dependent manner.
Iwai A, Marusawa H, Matsuzawa S, Fukushima T, Hijikata M, Reed JC, Shimotohno K, Chiba T.
Oncogene 2004 Sep; 23(45):7593.
Application：WB, Human, MCF-7 cells.
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Siah-1L, a novel transcript variant belonging to the human Siah family of proteins, regulates beta-catenin activity in a p53-dependent manner.
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