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Last updated: 2017/1/22
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PTGS1 polyclonal antibody

  • Catalog # : PAB6095
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of PTGS1.
  • Immunogen:
  • A synthetic peptide corresponding to human PTGS1.
  • Sequence:
  • C-QDDGPAVERPSTEL
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 68.7, 64.5
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize both human isoforms of this protein according to NP_000953.2 and NP_542158.1.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:32000)
    Western blot (1-3 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • PTGS1 polyclonal antibody (Cat # PAB6095) staining (1 ug/mL) of HeLa lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • PTGS1 polyclonal antibody (Cat # PAB6095) (3.8 ug/mL) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5742
  • Protein Accession#:
  • NP_000953.2;NP_542158.1
  • Gene Name:
  • PTGS1
  • Gene Alias:
  • COX1,COX3,PCOX1,PGG/HS,PGHS-1,PGHS1,PHS1,PTGHS
  • Gene Description:
  • prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
  • Gene Summary:
  • Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes PTGS1, which regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. PTGS1 is thought to be involved in cell-cell signaling and maintaining tissue homeostasis. Alternative splicing of this gene generates two transcript variants. The expression of these two transcripts is differentially regulated by relevant cytokines and growth factors. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000022052,OTTHUMP00000022053,prostaglandin G/H synthase and cyclooxygenase,prostaglandin-endoperoxide synthase 1
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